Laboratory diagnosis of AFB
Samples suitable for laboratory testing
Getting suspect material tested for AFB spores can be a useful tool in eliminating AFB. Also samples of abnormal brood can be examined in the laboratory for the presence of AFB spores. It is also possible to test samples of bees or honey from beehives for the presence of spores. See more on sample types.
What the tests involve
Two tests are used. The first is a microscopic analysis, and is only used for samples of suspect larvae or pupae. The test involves making a smear on a microscope slide, fixing the smear to the slide using heat and carbol fuchsin stain, and then examining the smear for AFB spores using the oil immersion objective of a microscope. The total magnification required is 1000x.
The second test is a culture test, and involves growing out bacterial cultures of AFB. The test can be used with samples of adult bees, honey, or suspect larvae/pupae, and can be carried out in a home laboratory. A dilution of the sample is prepared, and then heated at 92°C for 20 minutes to kill all competing bacteria, but not the AFB spores. A small amount of the liquid is applied to a Petri dish containing sterile brain heart infusion agar, and then the plate is incubated at 37°C for three days. The plate is then inspected for the presence of AFB bacterial colonies.
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Our videos cover everything from your legal obligations to how to recognise AFB, collecting cell and bee samples and more.
There’s a lot of good information here, telling you everything you need to know about recognising AFB: the visual symptoms, smell of AFB and more.
New Zealand beekeepers have a number of legal obligations that must be met regarding AFB disease. Read the shortened list in summary, here.
Most hives become infected because bees, honey or equipment have been put into a hive from another hive that is infected with AFB. Lower your chances of an AFB infection by reading this section.
Find out when the next AFB Recognition and Competency Courses, or Refresher Courses are available. These are held throughout the year in various New Zealand locations across the South Island and North Island.